Prevalence of BoHV-1 seropositive and BVD virus positive bulls on Irish dairy farms and associations between bull purchase and herd status

peer-reviewedBackground BVD and IBR are contagious viral diseases highly prevalent in Irish cattle. Despite their significant reproductive and economic impact very little is known about the BVD and IBR status of stock bulls (a bull used for breeding purposes). There are still a high proportion of dairy farms in Ireland that rely on the use of a bull for breeding cattle and ensuring the fertility of the bulls is of paramount importance for the efficiency of the farms. The prevalence of BoHV-1 and BVD in stock bulls in Irish dairy herds has never been investigated. The objectives of this study therefore were: (i) to provide descriptive, observational data on the use of stock bulls on Irish dairy farms; (ii) to investigate the BVD and BoHV1 status of a sub-set of stock bulls; (iii) to investigate factors associated with BVD and BoHV1 status of stock bulls and (iv) to investigate factors associated with dairy herd status for BVD and BoHV1, including any associations with the use of stock bull. A total of 529 blood samples from bulls involved in the dairy breeding process were analysed for BVD virus using RT-PCR, and BoHV-1 antibodies by ELISA test. A total of 305 different dairy herds took part in the study and the overall BVD and BoHV-1 herd status was determined by ELISA using four bulk tank milk samples over the 2009 lactation. Logistic regression was used to investigate the associations between the stock bulls and BVD and BoHV-1 herd and individual status. Results Of the 305 total participating farms, 235 farms (77 %) had at least one bull and 167 farms had purchased bulls. Two bulls (0.4 %) out of 529 tested were found positive for BVD virus and 87 (16.7 %) tested seropositive for BoHV-1. Some significant associations were identified between the purchase of bulls and both viral diseases. Purchased bulls were three times more likely to be seropositive for BoHV-1 than homebred bulls. In the same way, herds with purchased bulls were three times more likely to be classified as seropositive for BVD and four times more likely to have evidence of recent BoHV-1 circulation than farms where all the bulls were homebred. Conclusions The prevalence of BoHV-1 and BVD in stock bulls in Irish dairy herds has never been investigated. This study highlights the widespread use of stock bulls in Irish dairy herds, as well as the high rate of exchange of bulls between farms. Significant associations were found between the origin of the bull and their serological BoHV-1 status. In keeping with these results, bulls with higher number movements between farms were more likely to be seropositive for BoHV-1

Insect Management in Seed Corn Production

Corn grown for seed is often affected by the same pests as are found in corn grown for grain. However, integrated pest management (IPM) decisions can be considerably different from those in grain production. This is because inbreds are typically less tolerant than hybrids to stress from pests and the value of the seed is considerably greater than corn for grain. European corn borer and corn earworm are probably the two most significant economic insect pests in seed corn production fields. Following is a summary of the highlights of research conducted at Pioneer Hi-Bred International and experience from seed production fields regarding key components of IPM decision making for these insects

The design-by-adaptation approach to universal access: learning from videogame technology

This paper proposes an alternative approach to the design of universally accessible interfaces to that provided by formal design frameworks applied ab initio to the development of new software. This approach, design-byadaptation, involves the transfer of interface technology and/or design principles from one application domain to another, in situations where the recipient domain is similar to the host domain in terms of modelled systems, tasks and users. Using the example of interaction in 3D virtual environments, the paper explores how principles underlying the design of videogame interfaces may be applied to a broad family of visualization and analysis software which handles geographical data (virtual geographic environments, or VGEs). One of the motivations behind the current study is that VGE technology lags some way behind videogame technology in the modelling of 3D environments, and has a less-developed track record in providing the variety of interaction methods needed to undertake varied tasks in 3D virtual worlds by users with varied levels of experience. The current analysis extracted a set of interaction principles from videogames which were used to devise a set of 3D task interfaces that have been implemented in a prototype VGE for formal evaluation

Playing at Sevens: The Impulse Load and Movement Demands of Men’s Collegiate Rugby

Rugby sevens is a widely practiced sport that combines high-speed anaerobic activity with positional specific play and many body impacts. Despite its popularity and its intense physical demands on the body, many of these demands have not been fully explored. An investigation into the specifics of these physical demands can aid coaches and athletes in training and preparation for future matches. The purpose of this study was to quantify the Impulse Load and movement dynamics placed on American rugby players during competition. With this data, position specific information was compared to determine differences in physical demands. Fourteen male collegiate rugby sevens players were assigned a microsenor device and bioharness prior to testing. Time motion analysis data was collected using these microsenor devices. Acceleration data was used to calculate Impulse Load and GPS data was used to quantify total distance and distance in six speed zones. The devices were placed on each player, securely fastened around the chest, and worn for the duration of the warm-up and two competitive matches, for a period of 1 hour and 47 minutes. Players were monitored using 5Hz global positioning systems (GPS) and 100Hz triaxial accelerometers. Players were further analyzed and divided by playing position (forwards and backs). The contrasts in physical demands based on position can provide feedback to coaches on position-specific training.Faculty Advisor: Brandi Eveland-Sayer

Evaluation of stroke early supported discharge services. Collaborative Leadership in Applied Health Research and Care (CLAHRC), Nottinghamshire, Derbyshire and Lincolnshire, UK

A Cochrane systematic review showed that Early Supported Discharge (ESD) following a stroke is both a safe and cost-effective alternative to continued in-patient management. Currently it is unclear whether the health and cost benefits established in the research literature still apply when ESD services are implemented in practice. Our study aims to evaluate the implementation of stroke ESD across three counties in the UK (Nottinghamshire, Derbyshire, Lincolnshire), and offers an innovative approach to facilitate the translation of stroke rehabilitation research into clinical practice

Oxford Nanopore sequencing, hybrid error correction, and de novo assembly of a eukaryotic genome

Monitoring the progress of DNA molecules through a membrane pore has been postulated as a method for sequencing DNA for several decades. Recently, a nanopore-based sequencing instrument, the Oxford Nanopore MinION, has become available, and we used this for sequencing the Saccharomyces cerevisiae genome. To make use of these data, we developed a novel open-source hybrid error correction algorithm Nanocorr specifically for Oxford Nanopore reads, because existing packages were incapable of assembling the long read lengths (5-50 kbp) at such high error rates (between approximately 5% and 40% error). With this new method, we were able to perform a hybrid error correction of the nanopore reads using complementary MiSeq data and produce a de novo assembly that is highly contiguous and accurate: The contig N50 length is more than ten times greater than an Illumina-only assembly (678 kb versus 59.9 kbp) and has >99.88% consensus identity when compared to the reference. Furthermore, the assembly with the long nanopore reads presents a much more complete representation of the features of the genome and correctly assembles gene cassettes, rRNAs, transposable elements, and other genomic features that were almost entirely absent in the Illumina-only assembly

Understanding the role of the stroke physician in early supported discharge services. Collaborative Leadership in Applied Health rRsearch and Care (CLAHRC), Nottinghamshire, Derbyshire and Lincolnshire, UK

Our recent research, using expert consensus with trialists who had contributed to the Cochrane systematic review, has helped to establish key components of effective Stroke Early Supported Discharge (ESD) services. One essential element is that the ESD multidisciplinary team should have access to specialist stroke knowledge. However, how this is organised and put into working practice is less well-defined